Objective: The aim of this article is to compare the efficacy of Herbal irrigants versus Sodium hypochlorite (NaOCl) and Ethylene diamine tetra acetic acid (EDTA) on smear layer removal by using scanning electron microscope. Methods: 40 extracted single rooted teeth were randomly divided into 4 groups. Different irrigants were used for each group.17% EDTA & 3%NaOCl for group1, Triphala for group 2, Azardirachta indica (Neem) for group 3 and Curcuma longa (Turmeric) for group 4. Teeth canal were flooded with 1ml irrigant of respective group. After instrumentation, final irrigation was also done with 2ml of respective irrigant for two minutes for smear layer removal followed by irrigation with 2ml of saline and dried. All samples were analyzed using SEM for smear layer removal. Efficacy of smear layer removal was calculated using median (Interquartile range). Results: EDTA (17%) & NaOCl (3%) were most efficient in smear layer removal at all the levels with median(IQR) i.e. at coronal 1(0), middle 1(3) and apical level 3(1) respectively compared to Triphala ,Turmeric and Neem. Kruskal–Wallis test revealed statistically significant difference in smear layer removal by root canal irrigants at coronal third but no significant difference at middle and apical third. Conclusion: Triphala, Neem and Turmeric showed the potential to remove the smear layer. However EDTA and NaOCl showed the maximum efficacy in removing the smear layer.