Developing a low cost, high-efficiency and environmentally friendly approach to synthesize Silver Oxide nanoparticles for biomedical applications has become a highly research focus in the current scenario. In the present investigation, the AgO NPs were synthesized via a green process using different plant extracts such as Vitex Negundo leaf extract and Eucalyptus leaf extract. X-ray diffraction (XRD), Scanning electron microscopy (SEM), Energy dispersive x-rayanalysis (EDX), and Fourier Transform Infrared Spectroscopy (FTIR) were used to investigate the evolution of the size, morphology, chemical composition and functional group of synthesized AgO NPs. Antibacterial efficiency and Cytotoxicity studies were carried out for the green synthesized AgO NPs using both extract and compared their efficiency.