Honey is a functional food with antibacterial, anti-inflammatory, and antioxidant properties. The aim of this study is that Honey-mediated green synthesis of nanoparticles has been proven as a fast, safe, biocompatible, and cost-effective method. These three nanoparticles were prepared by the green synthesis method. Honey acts as both a stabilizing and reducing agent in the NP synthesis process and serves as a precursor in the green synthesis sol-gel method. Nanoparticles offer strength and medical imaging applications. In this study, we compare the three metal nanoparticles—green synthesized ZnO NPs, Cu-NPs, and AgNO3 NPs—with Indian sunflower honey on the basis of their particle sizes and antibacterial activity. The observed particle size of honey ZnO, Cu & AgNo3 NPs were 117nm,94 nm, and 64nm with great thermal stability. The products were characterized using UV–visible (UV–vis) spectroscopy, X-ray diffraction (XRD), Field-Emission Scanning Electron Microscopy (FESEM), and Fourier Transform Infrared spectroscopy (FTIR). The antibacterial activity results of Ag NPs seen against Gram-positive (S. aureus) and Gram-negative (E. coli) Bacteria were significantly more than Zn and Cu NPs.Our research leads us to conclude that AgNPs are superior compared to ZnO and CU nanoparticles synthesized from sunflower honey. The result obtained for this study is useful for researchers working on these three metal NPs syntheses from Sunflower honey for their formulation and various herbal synthesis products to select the suitable NPs in their work according to the size of NPS, characteristics fit, and antibacterial activity