The Eastern Ghats region, encompassing areas such as Visakhapatnam, Paderu, Minimuluru, and Vanjangi, is known for its rich biodiversity, including various fungal species like mushrooms. These mushrooms play a crucial role in the ecosystem as decomposers and are of interest for their potential culinary, medicinal, and ecological value. The present study focused on the comprehensive screening of mushrooms within these specific areas. Extensive field surveys were conducted to collect samples from diverse habitats, including forests, grasslands, and agricultural lands. The samples were carefully identified using both morphological characteristics and molecular techniques to ensure accurate species identification. The study revealed a diverse array of mushroom species inhabiting the Eastern Ghats soil. Species such as, Agrocyve, Agaricus, Psilocybe, Panaeolus, Agrocybe, Coprinopsis, Lentinellus, Daedaleopsis, Ganoderme, Coprinellus were among the identified genera. The mushrooms exhibited varying morphological features, including cap shapes, colors, and sizes, as well as distinct gill patterns and stem characteristics. Molecular analysis provided further insights into the genetic diversity and relationships among these species. Importantly, some of the identified mushrooms are of significant economic importance. Edible species like Pleurotus offer nutritional value and have potential for cultivation. Additionally, certain mushrooms have medicinal properties and are traditionally used by local communities for various health purposes. This study contributes to the documentation of mushroom diversity in the Eastern Ghats region and highlights its ecological importance. The findings underscore the need for conservation efforts to preserve these valuable fungal resources and their habitats. Furthermore, the identification of edible and medicinal species opens avenues for sustainable livelihoods and pharmacological research.