Aim: The current study aims to compare the incidence and severity of postoperative pain following root canal filling with calcium silicate-based (Endoseal MTA sealer) and epoxy resin-based (AH Plus sealer) sealers. Materials and Methods: 60 patients with asymptomatic irreversible pulpitis were randomly assigned into two groups based on the type of obturating sealer: the AH Plus sealer and the EndoSeal MTA sealer. These groups were then divided into two subgroups based on the number of visits, with subgroup A receiving a single-visit root canal treatment (RCT) and subgroup B receiving a two-visit RCT. After obturation, the postoperative pain was assessed using a visual analogue scale. Results: The present study was designed for the evaluation of incidence and intensity of pain after obturation using epoxy resin-based root canal sealer (AH Plus sealer) and calcium silicate-based sealers (Endoseal MTA sealer) in single visit and two visits treatment. Conclusion: The two types of sealers examined did not have any significant impact on the incidence of postoperative pain. However, it was observed that the single-visit endodontic therapy resulted in higher levels of pain during the first three days compared to the two-visit therapy approach.