Through scientific literature, wound healing represents an important medical problem. It is a complex biological process. This work might draw attention to the effective and promising role of BM-MSCs, extracellular vesicles and PRP on histological, immunohistochemical and biochemical evaluations of the three regimens on healing of skin wound. This study carried out on 60 albino male rats; a full-thickness skin square wound (1.5x1.5 cm2 ) wound was created on the middle of the dorsum of the trunk in the rats. BM-MSCs, extracellular vesicles (exosomes that derived from stem cells) and PRP were injected intradermal into the four corners of the wound. On the 7 th and 21st post-operative days, biopsies were taken to be examined under a light microscope at both stages of wound healing, allowing for a sequential evaluation of wound healing. Our work proved that BM-MSCs, extracellular vesicles and PRP showed improvement in regeneration of the epidermis and dermis in all healing process stages. Regarding the inflammation and angiogenesis, the treated groups showed moderate degree of inflammation and neovascularization especially exosomes treated group. Epithelization improved in all treated groups especially the stem cells treated group. Also collagen formation and deposition enhanced in all treated groups especially stem cells and exosomes treated groups. Elasticity of the skin was improved by the three modalities.