Kigeliaafricana bio-oil is potential feedstock for the production of biodiesel. This bio-oil could be efficiently extracted so that the feedstock cost could be reduced. A comparative study on the extraction process have been studied using Soxhlet extraction, aqueous acid extraction, two phase solvent extraction and ultrasonic assisted extraction process. The yield of oil and acid value was the two parameters focused. The highest yield of oil was observed for the Soxhlet extraction method with 45.1% and the lowest acid value was observed for the two-phase solvent extraction method as 4 mg KOH. The physiochemical properties of the oil were studied and for all the methodologies it was found to be within ASTM standard limits.