Candida albicans is the most common fungus isolated from failed endodontic cases. Due to the inherent disadvantages of sodium hypochlorite, researchers have looked for herbal alternatives. Thus, the present study is aimed to explore newer irritants which will probably be as effective or more and at the same time would be able to overcome the drawbacks of NaOCl. Methodology- 0.2% chlorohexinate Gluconate showed a moderate zone of inhibition of all microbial inhibition stains. Extracted human mandibular premolar were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to Candida albicans grown on Saboraud Dextrose Agar to form a biofilm. At the end of 2rd week, all groups were treated with test solution and control for 10 minutes and evaluated for Candida growth and number of colony forming units. Results &Conclusion- Propolis performed equally well as that of NaOCI and while chlorohexidine showed moderate results.