Introduction: Formation of White Spot Lesions is one of the common side effect during long term Orthodontic treatment. Accumulation of plaque and calculus around the bracket is the key factor for its formation. There are several ways to control its formation. Present invitro study evaluates the efficacy of three different types of varnishes in preventing enamel demineralization around orthodontic brackets. Methods: 80 freshly extracted premolars were collected for the study. After inclusion and exclusion criteria 72 teeth were selected and divided into 4 groups with 18 teeth in each group. Group 1 is control were no varnishes is applied, Group 2 contains teeth which was coated with 5% sodium fluoride varnish (voco proflurid), Group 3 contains teeth which were coated with ACP-CCP varnish (GC MI), Group 4 contains teeth which were coated with Nano silver fluoride varnish (NSF). After demineralization cycle the teeth were ground sectioned using hard tissue microtome. The teeth were then analyzed under polarization microscope for the depth of enamel demineralization. Results: Increased amount of enamel demineralization was found in Control group (32.26±11.61µm) Followed by Group 2 (27.19± 13.15µm) , Group 3 (28.22±14.5µm). The Group 4 showed least amount of enamel demineralization (12.1±5.1µm). There was a statistically significant difference in the measured depths of enamel demineralization in control group when compared with other groups (p=0.00). Conclusion: The NSF group (group 4) showed least amount of depth of enamel demineralization. Hence Nano Silver Fluoride can be used as varnish for preventing formation of white spot lesions around Orthodontic brackets instead of conventional fluoride varnishes.