This research was carried out with the aim of knowing students perceptions of the use virtual reality laboratory as learning media in chemistry practicum activities. This research is a type of descriptive quantitative research which was carried out in several National Senior High Schools (SHS) in East Jakarta area which already has a real chemistry laboratory with good facilities beside a national standard. The research sample was selected using a proportional random sampling technique. This research sample consisted of 150 students from national SHS 100, national SHS 71, and national SHS 42 Jakarta. The research instrument used was a test instrument in the form of questionnaire consisting of 15 statements. Instrument were arranged using a likert scale with a range of 1-5. Before use it, the research instrument was first validated by an expert validator. Data analysis was carried out using the one-way anova test with a significance level of 5%. Based on the result of data analysis, it is known that there are significant differences in perceptions regarding the use of virtual laboratories as a media for carrying out practicums with a value of Fcount (8.215) > Ftable (3.697). It is further known that the percentage level of student perception at national SHS 100 is the most positive compared to national SHS 71 and national SHS 42.