There are many thousands of types of bacteria and microorganisms found in aquatic ecosystems, including your personal pond. The main aquatic groups include arthropods, bacteria, protozoa, hydras, fungi, phytoplankton, and algae. A pond is a small area of still, fresh water. Bacteria rarely live alone but in communities with other bacteria. This is true both in the environment and in and on our bodies. Identification and characterization of microorganisms is a key part of the management of food safety and quality, tracing contaminants and troubleshooting problems such as spoilage. Knowing how to identify bacteria or other unknown microorganisms that you have encountered can help to assess whether it poses a safety/spoilage concern or is likely to be heat resistant. Most of the currently known species of bacteria have been identified using traditional microbiological techniques such as the gram stain reaction, morphology, and metabolic reactions. The aim of this study was to isolate and identify pond bacteria