The New Analytical method was to develop and validate a rapid, sensitive and accurate method for estimation of Emtriva and Tivicay in drug product by liquid chromatography. The chromatographic separation was achieved on C18 or Phenyl column (Eclipse XDB-Phenyl 250*4.6, 5um) at ambient temperature .The separation achieved employing a mobile phase consists of 0.1%v/v Trifluoro acetic acid in water: Methanol (30:70). The flow rate was 0.8ml/ minute and ultra violet detector at 260nm. The average retention time for Emtriva and Tivicy found to be 3.595 min and 3.263 min. The proposed method was validated for selectivity, precision, linearity and accuracy. All validation parameters were within the acceptable range. The assay methods were found to be linear from 50.0 – 150.0µg/mL for Emtriva and 50.0-150.0 µg/mL of Tivicy.