Nurse performance is the result of work or a form of professional nursing activities for the achievement of a certain standard in providing health services. Work-family conflict will have an impact on nurses' performances. The purpose of this study was to determine the correlation of work-family conflict with the performance of female nurses in Manado Regional Public Hospital Bhayangkara Manado. This observational analytic research employs a cross-sectional approach. It was carried out in the inpatient room of the site area on February 12 to 28, 2022. The number of samples consisted of 38 nurses; they were selected from a total population of 61 female nurses. A proportionate stratified random sampling technique was used to select the samples. The results of the statistical test analysis showed p-value = 0.002 < 0.05, and the calculated chi-square value was greater than the value of the chi-square table (12.064 > 5.991), meaning that Ho was rejected and Ha was accepted. Accordingly, there was a correlation between work-family conflict and the performance of female nurses in the research site.