The screening and monitoring the petroleum related cytotoxicity in petrol pump workers can be a useful tool to detect initial cellular damage. Aim of our study is to assess the micronuclei frequency in buccal epithelial cells of petrol pump workers. In 80 exposed petrol pump workers, micronuclei frequency (MN) was assessed using PAP stain and compared with unexposed workers of age21-60 years. Statistical analysis was done using SPSS version 25.0. The Mann whitney U test was used to compare the mean MNC between the control and exposed group.MN of exposed population with habits of smoking and tobacco chewing showed significantly high frequency than the exposed group without habits. The MN was significantly higher in workers with longer duration of exposure .The micronuclei assay of exposed petrol pump workers paves a simple and easy method to identify patients with early genomic damage.