Objective: The study aims to investigate the wound healing response of the dug usnic acid and carrier graphene with its nano form. Method: Nano-precipitation method by sonication was adopted to formulate the nanoparticle. SEM test was performed to check the shape and average size of the nanoparticle. FTIR test was performed for the chemical interaction. Ointment was prepared by fusion method and the viscosity test was performed by Brookfield viscometer. Spreadability test was performed by slide method. In-vivo wound healing study and topical animal activity was performed to confirm the wound healing effect of the formulated nano-formulation. Excision wound model histology was done to confirm the activity. Statistical analysis was done by Anova. Results: SEM study shows that the particles is in the nano range and possess spherical shape. FTIR study shows no chemical interaction. The result of in-vitro drug release study shows that the nano-formulation posses higher drug release rate as compare to the drug alone. Topical drug administration is more suitable for the treatment of the wound healing, so the nano-formulation was incorporated into the ointment by geometric mixing. The viscosity and the spreadability test were performed on the different formulations of the ointment and the suitable one was selected for the topical administration. In-vivo wound healing study was performed on the Wistar albino rats for 16 days. Statistical analysis by Anova gives p<0.01. It was found that the usnic acid alone, and the nano form of usnic acid with graphene both possess wound healing activity but the nano-form with graphene possess more significant activity. Conclusion: The present wound healing study revealed that the nano-form of the drug possess higher wound healing activity than the normal formulation of usnic acid.