The quest for and development of combinational chemotherapeutic medicines with various modes of action and low side effects is an essential element of cancer treatment. Aside from developing wholly novel drugs with chemical properties that are obviously distinct from current ones, another strategy involves combining two or more pharmacophores into a single molecule. The method for making several 1,2,4-triazole derivatives is detailed in the scheme. By 5-Mercapto-3-pyrimidinyl-1,2,4 triazole with various substituted benzyl halides, a total of 7 distinct 1,2,4-triazole derivatives were obtained. Combustion Analysis, TLC, IR, MS, and other methods were used to confirm the physical and analytical properties of the newly synthesized 1,2,4- triazole derivatives. Compounds TP1-TP7 exhibit IC50 values ranging from 41.12 M to 61.11 M, with compound TP6 having the greatest activity against the murine melanoma (B16F10) cell line. TP 6 was discovered to have more strong anticancer action in anti-cancer screening. As a result, we believe that the findings of this study may pave the way for the creation of anticancer drugs with high efficacy and fewer side effects. As a result, 1,2,4-triazol-3-pyrimidine-based compounds may have a broad anticancer range and serve as a potential lead molecule in different malignancies.