A novel series 1,4-dihyropyridine via one-pot four-component reaction of 3-(p-substituted phenyl)-1-phenyl-1H-pyrazole-4-carbaldehyde, Malononitrile, diethyl acetylenedicarboxylate, and aryl amine to produce excellent yield without further purification. The product’s composition has been identified by spectrum studies. Synthesized substances were screened for in vitro antimicrobial activity. The substance’s antibacterial and antifungal activity was good in some cases.