The design of controlled delivery systems for a variety of applications connected to pharmaceuticals, food-related bioactive substances, agro-chemicals, etc., benefits from the versatility in polymer processing. Natural polymers often have a more complex chemical arrangement than synthetic polymers since they are produced by an organism or plant. The emulsion cross-linking approach was used to create casein nanoparticles. Copper oxide deposition inside the casein matrix was done in-situ to create copper oxide-loaded casein nanoparticles. With the use of XRD, FTIR, XPS analysis, and Raman spectroscopy, the interactions of nanoparticles of casein with copper oxide were investigated. The created nanoparticles have shown capacity as tiny vehicles that can transport various substances to specific sites. As we look ahead, these nanoparticles could play a crucial role in delivering loaded components to targeted areas.