MBL producing P. aeruginosa is an emerging threat and a cause of concern as they have emerged as one of the most feared resistance mechanisms. MBL belongs to Ambler Class B owing to their capacity to hydrolyze all β lactams including penicillins, cephalosporins and carbapenems with the exception of aztreonam. Aim& Objective:The aim of the present investigation was to characterize the prevalence of metallo β-lactamases and to study the antibiotic susceptibility profile among 102 clinical isolates of Carbapenem resistant Pseudomonas aeruginosa. Material & Methods: A total of 102 nonduplicate,consecutive, carbapenem resistant Pseudomonas aeruginosa isolated from patients hospitalized for 48 hours or more were included in the study.AST to imipenem and meropenem were determined and interpreted according to CLSI guidelines. The Combined disc test and MBL E test were used for screening of carbapenamases and MBL production respectively.PCR were performed for the detection of MBL (blaIMP,blaVIM and NDM) genes. Result:Among 102 Carbapenem (resistant to either or both Imipenem and Meropenem) non-susceptible isolates of Pseudomonas 27 (26.47%) were found to be MBL producers. Of 27 MBL-producing isolates, and 81.48% carried the blaVIM gene and 18.51% carried the blaIMP gene. All MBL-produc¬ing isolates were multidrug resistant.Conclusion: In our study the prevalence of MBL in P.aeruginosa which we found to be 26.47% of total carbapenem resistant isolates. Colistin and Polymixin B drug that showed 100% susceptibility towards MBL producing isolates. Timely identification of non-fermenters and monitoring their antibiotic susceptibility patterns are suggested for effective management of infections and limitation of the emergence of MDR.