Molecular interaction is the interaction between the two nearby molecules that are present in solute and solvent. In order to study about the molecular interaction between the solute aspartame and solvent malic acid, several acoustical parameters are calculated by using basic parameters. Basic parameters such as ultrasonic velocity, density, and viscosity are measured, from which various acoustical parameter are calculated and interpreted. Spectroscopic studies are made to find the functional group and band gap. The results are interpreted and discussed. The applications of the binary mixtures are also interpreted.