The aim of this research is to study the effectiveness of Pseudomonas aeruginosa bacteria isolated from hospital environment samples to produce Protease, Lipase, Amylase, Urease, DNase and Hemolysine. One hundred samples were were collected from Al-Alam Hospital during July 2020 till the end of January 2021 to isolate and identify P.aeruginosa from different environmental sources in the hospital including the operating theaters, patients' beds and surgical kits. Twenty eight isolates were found to be belonging to P. aeruginosa, which mean an isolate rate of (28%) of the total samples. The diagnosis was carried out based on phenotypic characteristics and biochemical tests. P. aeruginosa was found in (11) out of (41) operating theaters samples, (9) out of (32) hospitals' beds samples and (8) out of (27) surgical kits samples. This means that isolates from the surgical kits constituted the highest percentage of (29.6%) of the total samples, while the lowest percentage of (26.8%) was obtained from the operating theaters samples. The results showed that (35.7%) of total isolates were Hemolysin producer on blood agar, It was also found that (57.1%) of total isolates were Amylase producer on Starch agar, (67.8%) of total isolates were Protease and Lipase producer on Skim milk agar and on Rhan agar, (21.4%) of total isolates were Urease producer on Urea agar and (53.5%) were DNase producer on a DNase agar medium