Aegle marmelos leaves and Pedalium murex has been used as a remedy for gastrointestinal infections, antidiarrheal, anti-inflammatory, anticancer, antidiabetic and antioxidant activity of human beings. Extraction was obtained using ethanol and fractionation in column chromatography with different solvent followed with: beginning with chloroform, ethyl acetate, methanol and water. The invitro antioxidant activity, total flavonoid content and Total polyphenol content nine fraction of Aegle Marmelos and Pedalium murex leaf extract was determined by using spectrophotometric method. The all fraction obtained from extract were based on solvent concentration for nine fractions. The total antioxidant capacity of the fractions was calculated in mg/ml of ascorbic acid with range between 48-132 mg/ml for Aegle marmelos and 43.3-116.3 mg/ml for Pedalium murex, total flavonoid content of the fractions was expressed of quercetin equivalent (QE) ranging between 47.5 – 83.5 mg/ml for Aegle marmelos and 112.6-146.3mg/ml for Pedalium murex, and total phenolic content of the fractions were expressed of Gallic acid equivalent (GAE) ranging between 108.2 – 181.5 Aegle marmelos and 152.6-211.5 for Pedalium murex. All the fractions, it was observed that methanol water fraction was having better antioxidant property