A wart is the type of solid skin growth caused by the human papilloma virus (HPV). Objective: The aim of the present investigation was to formulate and standardize an Ayurvedic Preparation for surface warts. Patients and Methods: This clinical trial study are selected on people (Older than 10 years) living in Shirpur city, of Maharashtra, who had warts on their skin in 2018. The sample size will be 50 patients, 25people in the Ayurvedic formulation group and 25 in the placebo group. Group the participants were randomly divided into two group using4-block sampling. Photographs were taken from the warts of Intervention group using digital camera. Then formulation was applied on wart skin and instructed the patient repeated for Three month. All stages in the intervention group were similar to the placebo group. Placebo was used only linseed oil Patients’ wart status in both groups was examined Two week and one month after by the physician in terms of improvement or lack of improvement. Data were analyzed using PSPP software version 18, chi-square test, Fisher’s exact test, Mann-Whitney test and ANOVA for repeated measure. Result: Based on the results of the above characterization methods, the optimized batch was selected and was further subjected to a clinical trial on a human volunteer with consent and stability studies at 30 ± 2 0C and 75 ± 5 % RH. The optimized formulation was found to reduce the wart significantly. Conclusions: As peoples are unaware about the complications of warts with the increasing age (particularly in females) and compliance with body (non-harmful), majority of them ignore this problem, As it can cause serious health issues, removal of the same is at most important at early age, This research will give a promising breakthrough for the removal of warts which will be benefited to the society in near future.