Betulinic Acid, a triterpene found in nature, has potent anticancer effects. However, it has limited solubility in the aqueous phase as a result of its poor bioavailability. Damar Gum nanoformulations are a practical method to give Betulinic Acid better solubility and bioavailability. Betulinic Acid Damar Gum nanoparticles (BDNs) were made in the current study, and their physical and chemical properties were examined and modified. BDNs demonstrated the highest qualities for usage in drug delivery applications with a particle size range of 48-135 nm, a zeta potential of -25.1 mV, and a percent entrapment effectiveness of 68.7%. The cytotoxic efficacy of the BDNs against the A-549, MCF-7, and Hela cell lines revealed its capacity to prevent cancer cell proliferation. The current study project demonstrated how BDNs could be employed as promising nanoformulations for cancer treatment.