The nano emulsion of ethanolic leaves extract of P. wallichiana has not been formulated and tested for anti-fungal activity using prescribed protocols and methods. Therefore, this research focuses on development and characterization of P. wallichiana based nano emulsion used against fungal infection. Ethanolic leaves extract of P. wallichiana was obtained from the Uttarakhand region and other chemicals from Sigma Aldrich, India. It was identified and authenticated by a botanist. The powder of P. wallichiana were weighed and extracted through Soxhlet apparatus using ethyl alcohol. Pre-formulation study was performed for estimation of better compatibility profile. After that total 6 formulations of nano emulsion were developed. Formulations were evaluated for parameters including physical appearance, droplet size & polydispersity index, in-vitro drug release, % drug content, viscosity, pH and stability. It results, P. wallichiana has more anti-fungal potential on A. flavus as compared to A. niger. It might be due to destruction of cell wall or nucleic acid of fungi. In terms of pH, % drug content, and in-vitro drug release, all stability criteria were nearly the same after 1 month as they had been in prior evaluations. In conclusion, NE 5 was most prominent nano emulsion among other preparations. It also showed improved stability, maintaining its pH and medication release rate during a month of storage. It showed a remarkable anti-fungal potential against both the fungal species used. After in-vivo clinical study, this formulation could be used to treat and distribute drugs for conditions including cancer. Dermatitis, pruritis, and other epidermal illnesses can be treated with this.