The uncontrolled excessive usage of insecticides in agriculture to manage pests leads to considerable side effects such as resistance, regeneration, and residue. The present study aimed to evaluate the toxicity of environmentally-safe mixtures pesticides belonging to different groups (Extreme 36% SC (methoxyfenozide 30% + Spinetoram 6%), Atifos-super 55% EC (chlorpyrifos 50% + cypermethrin 5%), and Tempo-xl 30% EC (chlorpyrifos 25% + lufenuron 5%) against the 4th instar larvae of Agrotis ipsilon using leaf-dip method. The current data indicated that Tempo-xl recorded the highest efficacy against treated larvae with LC50, followed by Extreme and Atifos-super (4.09, 05.03, and 9.78 ppm, respectively). Moreover, the percentage of pupation, adult emergence, growth index, fecundity, and egg hatching exhibited a significant decrease; also, the larval and pupal weights detected a significant reduction. The biochemical analysis showed that the tested compounds significantly enhanced the total protein, α- and β- esterase, acetylcholinesterase, protease, and chitinase. Therefore, Tempo-xl could be recommended as an eco-friendly alternative to synthetic insecticides for the appropriate integrated pest management (IPM) program of A. ipsilon.