In order to determine whether Vitex negundo leaf extracts have anti-arthritic efficacy in rats, the study's goal is to create and test a topical herbal gel comprising the extracts. The physical appearance, net content, viscosity, extrudability, pH, spreadability, in vitro diffusion profile, and primary skin irritation tests of six herbal gel formulations made with 1.5% of the gelling agents carbopol 934 (F1-F6) were assessed. According to ICH recommendations, a stability study was completed for the topical herbal gel formulation, and Freund's Complete Adjuvant (FCA) induced arthritis technique was used to assess the anti-arthritic activity. Haematological and biochemical markers were also evaluated, along with body weight and paw volume. The gels that were created adhered to the rules and were homogenous and stable. F4 outperformed the other formulations in terms of release characteristics (84.37%). The skin irritation test revealed no erythema or edoema, indicating the gel is safe and nontoxic. In comparison to rats with the disease, topical administration of the herbal gel F4 containing carbopol 934 demonstrated considerable (p 0.001) anti-arthritic action. The anti-arthritic activity of the gel formulation was substantiated by a decrease in paw volume, no agglutination of C-reactive protein and rheumatic factor, a decrease in TNF level, and a return to normal haematological and biochemical parameters.