The main objective of developing stable mucoadhesive formulations of itraconazole using natural and synthetic mucoadhesive and rate retarding polymers is to treat fungal infections, particularly oral candidiasis, and to investigate the effect of different polymers on drug release profiles for prolonged release. The rheological parameters of the powder bed, such as bulk density, compressibility index, and angle of repose, were assessed. Mucoadhesive buccal tablets were compressed on a Chamunda, Pilot Press II D-B with 8 mm flat faced punches, and every batch was evaluated for weight variation, hardness, thickness, percent swelling index, mucoadhesive strength, and in vitro drug release using a USP TDT-081 dissolution testing apparatus, method II with a paddle at 50 rpm and a kinetic study. In addition, the prepared mucoadhesive formulations were evaluated for a number of quality control tests, antifungal efficacy and stability studies, which were conducted at 40°C/75°RH in a stability chamber for a period of six months.