The present study framed the design and development of a nanosuspension of Vitamin D3 (VD3) for oral drug delivery. VD3 nanosuspension was prepared by oil-water emulsion process by using Eudragit polymer and poloxamer as a stabilizing agent. Its physicochemical properties were studied by Differential Scanning Calorimeter (DSC) and Fourier Transform Infrared Spectroscopy (FT-IR). The mean particle size of drug-loaded NPs obtained by a particle-sized analyzer was 92 to 200 nm. High encapsulation efficiency (>85%) showed that nanosuspensions were stable. In vitro, release study shows successful steady release of VD3 from nanosuspension. Almost 98% of VD3 was released from nanosuspension, which is 30% more than existing marketed formulations.