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ISSN 2063-5346
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DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR QUANTIFICATION OF CURCUMA LONGA AND DALBERGIA SISSOO IN BULK AND DOSAGE FORM USING UV-VISIBLE SPECTROPHOTOMETRY

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Vishnu Das, Joohee Pradhan
» doi: 10.53555/ecb/2022.11.11.123

Abstract

Background: Curcuma Longa, and Dalbergia sissoo, are used traditionally as an antiinflammatory, antipyretic, analgesic, anti-oxidant, anti-diabetic, and antimicrobial agent and many other medicinal uses. No validated method currently exists for the estimation of Curcuma longa and Dalbergia sissoo, in combined dosage form. Objective: A simple, economical, sensitive, precise, and accurate UV-visible spectrophotometric method has been developed for the determination of curcuminoids and dalbergin from powdered extract of Curcuma Longa, (PECL) and Dalbergia sissoo (PEDS) as bulk and in a dosage form. Method: The quantitative determination of the PECL & PEDS was carried out using the UVvisible spectrophotometric measured at 428 nm and 273nm. The method was validated as per ICH guidelines. Statistical analysis of the result has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of curcuminoids and dalbergin in pharmaceutical formulations without any interference from common excipients. Result: The calibration curve was linear in the concentration range of 2-12 μg/ml and 20 to 120 μg/ml with correlation coefficient values of 0.9997 & 0.9982 for curcuminoids and dalbergin. The results of the analysis were validated statistically and by recovery studies. Conclusion: The proposed method was found to be a sensitive, specific, accurate, precise, and cost-effective quality control tool for the routine analysis of curcuminoids and dalbergin in bulk and in dosage form

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