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ISSN 2063-5346
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DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD FOR THE ESTIMATION OF ACECLOFENAC IN MARKETED FORMULATION

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Dr. Amjad Ali, Shakeel. A. choudhary, Sarkar Swagata Shyam, Mane Bhagyashri Shankarrao, Sayyed Mateen
» doi: 10.31838/ecb/2023.12.s3.712

Abstract

This is the study where we developed and validated an reversed phase high performance liquid Chromatography method with Ultraviolet detection for the Estimation of Aceclofenac drug in marketed formulation. The method was developed on kromasil C18 analytical column (150 X 150mm,4.6 μm) with mobile phase consisting of mobile phase A, buffer: methanol in ratio 90:10 and mobile phase B, ACN: methanol 90:10% v/v. The flow rate was kept at 1 ml/min and the column was maintained at 40°C. The detection carried out at wave length of 275 nm. Calibration plot was linear in concentration range of 50–150ppm levels with the correlation coefficient (r2) of 0.999.The method was validated with regards to system precision, method precision, linearity, accuracy, limit of detection, quantification and robustness and can be used for estimation of Aceclofenac drug in bulk and tablet dosage form. The forced degradation studies are also performed.

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