Background: Traditional knowledge (TK) can be described as an indispensable part of the identity of the ethnic communities. This knowledge can be of different types including the TK of medicinal plants. However, with the advancement of allopathic medicines, this knowledge is deteriorating. Objective: The main objective of the current study is to record the traditional use of plants for treating diseases or conditions in humans in the lower Brahmaputra Valley zones of Assam. Materials and methods: A survey was conducted using semi-structured questionnaires to collect data on plant-based medicines from among 25 traditional healers from the various districts of lower Brahmaputra Valley zones through face-to-face interviews and discussions. The acquired information was compiled through the UR (Use report). The scientific name of the plants was verified using WFO (https://wfoplantlist.org/plant-list).