Introduction: One of the most dreaded effects of Diabetes Mellitus (DM) are diabetic foot ulcer (DFUs), which frequently become infected and result in problems like osteomyelitis, amputations, and septicemia. There are few accounts of information about the costs and patterns of antibacterial therapy for DFUs. Objective: The objective of this study is to analyze the pattern of drug use in patients with diabetic foot ulcer in a tertiary care teaching hospital, in Chennai. Methods: A prospective observational study was carried out in the department of General surgery at a tertiary care teaching hospital, Chennai. Demographic details and treatment data of 50 patients were collected in a specially designed data collection form, and the data were analyzed using Microsoft Excel. Results: According to Wagner's classification, patients admitted with DFUs predominantly belonged to grade 3 category (50%), followed by grade 2 (28%) and grade 4 (22%) categories. A total of 50 organisms were obtained from the specimens. The most common isolates were Staphylococcus aureus (66%) and Pseudomonas aeruginosa (18%) followed by E. coli (10%). Cefotaxime was the most commonly prescribed empirical antibiotic (48%), followed by piperacillin-tazobactam (42%), Conclusion: The majority of the organisms that were isolated from DFUs were resistant to various medicines. Piperacillin+ Tazobactam and linezolid were mainly effective against Gram-negative organisms, while cefotaxime and metronidazole were more effective against Gram-positive organisms. It's crucial to heal wounds locally. It highlights the importance of early detection, appropriate wound care, appropriate antibiotic therapy, and optimal glycemic control in the prevention and management of this complex condition.