Concept mapping one of the new innovative teaching strategies which is very important and critical to integrate into nursing education that improve students’ decision-making, clinical judgment, and academic achievement of students. The aim of this study was to assess the effect of concept mapping on knowledge, attitude, and academic achievement of nursing students. Methods: The study was conducted at the College of Nursing, ABHA, King Khalid University, Kingdom of Saudi Arabia, using a quasi-experimental design. Simple random sample from 2nd year nursing students (n=60). Four tools were used to collect the data: knowledge questionnaire sheet, rubric for assessing concept map, attitude assessment tool, and students' achievement test. Results: The study findings indicate that there is improvement in the concept map knowledge of nursing students after awareness sessions, the highest percentage of nursing students achieve high level in final written exam and had a positive attitude towards concept mapping as learning tool. Conclusion and Recommendation: The study findings add to the literature supporting the use of concept map in nursing education. The approach is beneficial for enhancing nursing students' knowledge about concept map and academic achievement. Therefore, the study recommends that the nursing educational programs should introduce this innovative teaching learning strategy in different nursing courses.