One of the most prevalent neurological disorders is multiple sclerosis (MS). In this condition, the immune system assaults the central nervous system's oligodendrocyte cells and myelinated neurons, destroying them. These ailments cause poor nerve impulse conduction and show symptoms like weakness, exhaustion, and visual and movement problems. The purpose of this study was to determine whether the ethanol extract of Alternanthera sessilis Linn. (EEAS) could mitigate the behavioural and histological alterations in male rats brought on by Cuprizone. By administering 0.4% Cuprizone (CPZ) by oral gavagefor six weeks, demyelination was induced. For the latter two weeks of treatment, EEAS 200mg/kg was administered once daily. In comparison to the control group, therapy with CPZ caused weight loss over the course of 6 weeks, while EEAS administration stopped. A decline in motor coordination and balance was seen in the group receiving CPZ treatment in behavioural tests (pole test, narrow beam test and y-maze) (P 0.01). These motor impairments have improved with EEAS treatment over the past two weeks. The CPZ group showed increased demyelination, which was reduced by the use of EEAS, according to histopathological investigation.