Euphorbia miliihas been traditionally used in Indian medicine as a result of its curative results of hepatitis, gonorrhea and diabetes; it is probably not proof-founded. However folklore has given us many powerful therapies, based on plant sources. So claims which can be made for the protective efficacy of Euphorbia milii (family: Euphorbiaceae) to treat hepatic diseases. The present study focused on investigating the role of alcoholic extract of Euphorbia miliifor its antioxidant activity such as DPPH and FRAP scavenging activity. The in vitro cytotoxicity test was performed to determine the toxicity effect as per ISO guideline and found to be non-toxic. The plant extract (AEEM) appreciably prevented the increased in serum Aspartate amino transferase (AST), Alanine amino transferase (ALT), alkaline phosphatase (ALP) and total serum bilirubin (SB) level in acute liver damage by paracetamol and elevated the activities of lipid peroxidation (LPO) and glutathione (GSH) in the liver. Histopathological observation of the liver used to be additionally performed to further support the evidence from the biochemical analysis. The observation that these significant protective effect against acute hepatotoxicity induced by paracetamol of Euphorbia milii.