Indians frequently use the well-known medicinal plant Withania somnifera (L) Dunal to treat a wide range of clinical conditions. Asgand, a common chemical, has long been employed in the Unani and Ayurvedic medical systems, either alone or in conjunction with other therapies. It is mentioned in Discords' book "Kitabul-Hashaish" (78 AD). Withania somnifera is a plant that has anti-inflammatory (Mohallil-e-Warm), sedative (Musakkin), alterative (Muaddil), and aphrodisiac (Muqawwi-e-Bah) characteristics. Asgand is frequently discovered in the roots and leaves of this plant. A concerted effort has been made to look into a variety of topics related to the medication, including phytochemical and antibacterial research, keeping in mind the therapeutic properties of Withania somnifera. Since this plant is in danger of extinction owing to overuse, in vitro tissue culture is one of the greatest ways to preserve it and generate several Withania plants (from any part, fragment, or cell of plants due to totipotency) in a small space and at any time of year.