Purpose: To determine the effect of intraperitoneal Platelet Rich Plasma (PRP) Heterologous on hepatocyte mitotic index of Wistar white rats after partial hepatectomy. Methods: This is an experimental study with a post-test only control group design conducted at the INA Lab Padang Laboratory from April-May 2023 which recruited 8 weeks old male Wistar white rats (Rattus norvegicus) with a body weight of 200- 300 grams. The rats were divided into sham surgery, controls group (Partial Hepatectomy without PRP), and animal trial (partial hepatectomy with PRP). Results: There were 18 rats divided into three groups. The mitotic index was found to be the highest in partial hepatectomy with PRP (0.60), followed by control group (partial hepatectomy without PRP (0.30), and the others (0.00). In this study, a significant association was found between intraperitoneal administration of PRP and hepatocyte mitotic index of Wistar white rats after partial hepatectomy (p=0.001). Conclusion: Intraperitoneal administration of PRP increases the mitotic index in the hepatocytes of white Wistar rats after partial hepatectomy