The ASHA workers possess the duty of providing information to the general public in rural areas regarding the basic aspects of health like nutrition, fundamental hygiene and sanitary practices, healthy conditions of life and work atmosphere, details regarding the prevailing health-related services and the requirement of timely usage of the welfare services regarding health. With reference to the services of the ASHA workers, the present study has been done to explore and analyse the impact of the knowledge of the ASHA workers over their performance. The respondents of the study are the ASHA workers in Kozhikode who have been selected through simple random sampling. The instrument used for data collection is a questionnaire and the tool used for analysing the collected data is Regression analysis. The sample size of the study is 100. The results of the study reveal that there is significant outcome (F = 36.304; p < 0.000) which means that the knowledge significantly influences ASHA Work performance.