Nowadays, silver nanoparticles have become crucial for biomedical purposes because of their overall anti-microbial potential effects. In this work, antibiotics, with and without silver nanoparticles, have been used in the nasal mucosa of rats. Nine rates were divided into three groups. The first group received oral amoxicillin/clavulanic acid at 80 mg/kg. The second group received oral amoxicillin/clavulanic acid at a dose of 80 mg/kg combined with silver nanoparticles at 50 mg/kg. The last group, as a control group, received distilled water and a standard diet for one month. At the end of the experiment, the rats were sacrificed, and tissues were removed from the nasal cavity. The results demonstrated that the silver nanoparticles conjugated with amoxicillin/clavulanic acid increased their distribution in the mucosa of the nasal cavity. The experimental results were evaluated using histological examination via scanning electron microscope. This investigational outcome is essential for many clinical studies of silver nanoparticles and their medical uses in otorhinolaryngology.