The field study investigated the effect of soil enzyme activity on tomato production under different irrigation regimes and various source of nitrogenous fertilizer. Soil enzymes play a crucial role in nutrient cycling and availability, thereby influencing the plant growth and productivity. The field experiment was carried out in a split plot design with two main plots having irrigation regimes and five subplots are different source of nitrogenous fertilizers. The results obtained from the experimentation indicated that soil enzyme activity was significantly influenced by the interaction of irrigation and nitrogen fertilizer. Higher soil enzyme activity (cellulase, urease and phosphatase activity) were observed in treatment combination of 0.6 IW/CPE ratio with vermicompost and neem coated urea fertilizer applied treatments. The lower value in the soil enzymes were observed in the nano-urea applied treatments. The present study results shows that higher irrigation will reduce the microbial activity in the soil and also the organic fertilizer like vermicompost will increase the soil microbial than other synthetic fertilizers. Additionally, optimal irrigation conditions positively influenced the soil enzyme activity leads to increase the tomato fruit yield.