Background: The role of yogic concepts is immense in stress management and other therapeutic benefits, but its results on antenatal mothers’ (AM) biomarkers are less researched. Objective: To test the influence of Asana, pranayama and dhyana on average blood glucose (BG) levels and hemoglobin (Hb) of the antenatal mother (AM). Methods: This Single blinded placebo-controlled trial was conducted at Saveetha Medical College and Hospital, Chennai, India. The samples were conveniently selected through consecutive sampling and allotted randomly using random number table into four groups namely group A (standard nursing care), Group B (Yoga therapy alone), Group C (antenatal exercises) and group D (Yoga therapy and antenatal exercises) with 33 samples in each group. The groups received their respective treatment for 2 days a week for 16 consecutive weeks. HbA1C and Hb levels were assed at baseline level (pre-test), and after 16th week (Post-test). Results: Totally 132 samples accounted for the study results, which showed that the group D subjects performed significantly well compared to the A, B and C groups in all temporal outcomes (P<0.01). Conclusion: Combining elements of therapeutic Yoga with antenatal exercises will be more effective in normalizing glycemic levels and improving Hb levels among AM.