To evaluate the effect of different levels with four concentrations of inclusion and a control (0), 4, 7 and 10 %) Creole macree zapallo seed flour (Cucurbita maxima duch) on the total cholesterol and fatty acids of the eggs of laying hens, 120 Indian hens of the vanaraja species were located for 80 days, according to a completely random design. Simple classification analysis of variance was applied, except for the ethereal extract concentration, where factorial analysis was used with four treatments and 20 repetitions. The concentration of ethereal extract in the egg increased due to the effect as the evaluated weeks passed and the different concentrations of HDSdZMC inclusion (32.97 to 37.19). The oilseed enriched the egg in octadecanoic, oleic, linoleic, and α-linolenic fatty acids; While it reduced the amount of arachidonic acid. The lowest ratio of saturated/polyunsaturated fatty acids and Om6/Om3 was observed, also the inclusion of this supplement decreased the total cholesterol in the egg with respect to the control. It is recommended to include 7 % to 10 % of Creole macre zapallo seed meal in the diets of laying hens to increase the ether extract, beneficial fatty acids, reduce total cholesterol and harmful fatty acids in the eggs of Creole laying hens, (fowl).