Aging and diseases associated with it develop because of free radicals and other reactive oxygen species causing oxidative stress and damage to the tissue, which has been implicated in cancer, atherosclerosis, other neurodegenerative diseases and diabetes [1, 2]. It is therefore required to measure it accurately. Oxidative stress is caused due to the over- production of the reactive oxygen species (ROS) and the disturbance developed in the antioxidant potential of bio- chemical processes [3, 4]. The ROS are hydrogen peroxide, superoxide anion, and hydroxyl radical [5, 6]. ROS mostly form in the brain due to high consumption of oxygen and insufficiency of endogenous antioxidant resistance mechanisms [7]. ROS is commonly generated by the mitochondrial electron transport chain (mETC), NADPH oxidases, xanthine oxidase, cyclooxygenase-I & II, and enzymatic stimulation of cytochrome P450 2E1 (CYP2E1) [8, 9]. CYP2E1 has an excessive percentage of NADPH oxidase activity, which causes the production of ROS and increases oxidative stress [10-14].