Methodology: A Quantitative research approach with Pre-experimental one group pre-test post-test design was used as research design for the study. 60 working mothers were selected by using convenient sampling technique. The data was collected by structured knowledge questionnaire. The collected data was organized in Master Data Sheet and analyzed by using Descriptive and inferential statistics as per the objective of the study. Results: The findings of the study revealed that in pre-test score, majority of mothers 51(85%) had moderate knowledge and 09(15%) had inadequate level of knowledge whereas in post-test score, majority of the mothers 53(88.3%) had adequate knowledge and 07(11.7%) had moderate level of knowledge regarding stress management. The obtained t & p value was t = -47.892, p =0.0001, which reveals that there was significant difference within both the groups at pre-test and post-test scores. There was no significant association between age, educational qualification, years of experience, types of family, source of information regarding stress management whereas there was significant association between religion. Conclusion: The study concluded that most of the mothers had adequate knowledge whereas only few mothers had moderate level of knowledge regarding stress management. Hence, it was concluded that structured teaching programme, is an effective teaching method to teach the participants and also help to enhance the knowledge of the working mothers regarding stress management.