Purpose: This study investigated the effects of Speed, Agility, and Quickness (SAQ) training on the biochemical parameters of under-19 (U-19) national-level elite soccer players. Materials and Methods: Fifteen elite soccer players with an average age of 16.87±1.13 years, an average weight of 61.87±5.94 kg, an average height of 172.82 ± 5.18cm, an average training age of 5.0±1.0 years, and intermediate participation in the national level competition for 4.0±1.0 years participated in the study. After baseline data collection, the participants underwent a twelve-week SAQ training program consisting of one hour of training per day for four days a week. Biochemical parameters, including red blood cells (RBC), Creatine, Hemoglobin, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL), were measured before and after the training program. The significance level was set at P<0.05. Results: The results showed a significant improvement in some segments of power performance in selected elite soccer players during the twelve weeks of SAQ training. Specifically, there was a substantial increase in RBC, Creatine, Hemoglobin, and HDL and a significant decrease in LDL and VLDL. Conclusion: The study findings revealed that prolonged SAQ training and physical activities impact the biochemical parameters of elite soccer players. Proper planning and implementation of SAQ training can enhance the performance of soccer players and help maintain the potency of performance.