Stem rot infection of peanut (Arachis hypogaea L.) encouraged by Sclerotium rolfsii Sacc. is acutely destructive disease in groundnut cultivated areas of the world. These examinations were executed to test the potency of aqueous extracts of 9 botanicals, 9 organic amendments and 9 antagonists against Sclerotium rolfsii Sacc inciting stem rot of groundnut in in-vitro during the period 2020-21. Among plant aqueous extracts of tested Garlic (Allium sativum) was recorded least fungal mycelial growth 0.20 mm and 0.10 mm at 15% and 20% concentrations respectively in contrast to control. The next greatest treatment against pathogen is C. longa (50.33 mm and 40.00 mm) followed L. camara (51.00 mm and 42.00 mm), E. globulus (72.66 mm and 60.33 mm), S. indicum (74.30 mm and 71.10 mm), M. pinnata (80.00 mm and 53.66 mm), Z. officianale (80.33 mm and 42.66 mm), A. cepa (87.33 mm and 61.00 mm) and A. indica (87.66 mm and 67.33 mm). In organic amendments fine decomposed Farm Yard Manure (FYM) and Groundnut oil cake were recorded maximum inhibited (100 percent) fungal mycelial growth for S. rolfsii followed by Mustard oil cake (88.88%), Sesamum (83.33%), Cotton (72.22%), Neem cake (70.36%), Karanj (60.74%) and Vermicompost (60.00%) which were importantly higher than control (0.00%). Bacillus subtilis which was most successful with least radial fungal mycelial growth (35.30 mm) with overhead mycelial zone of inhibition (60.70%) and least percent disease incidence (11.95) of the test organism and notably superior over all the biocides tested in in-vitro and in- vivo (pot assay) conditions respectively