Periodontitis is an infectious condition that causes inflammation of the tissues surrounding and protecting the teeth. It leads to the gradual breakdown of connective tissue, loss of alveolar bone, and in severe cases, tooth loss. Nonsurgical mechanical therapy serves as the cornerstone of periodontal treatment, playing a fundamental role in managing the disease and its progression. Aim: To evaluate the efficacy of Hyaluronic acid in combination with chlorhexidine and chlorhexidine mouthwash as an adjunct to Phase-I therapy in periodontitis patients. Methodology: A total of 10 periodontitis patients were enrolled and randomly divided into 2 groups of 5 each by coin toss method. Group A and group B were treated with 0.025% hyaluronic acid+Chlorhexidine gluconate 0.2% and chlorhexidine gluconate 0.2%, respectively, along with phase I therapy. Patients were directed to use 10ml of the respective mouthwash, twice daily for 21 days. A pre-oral and post-oral saline rinse of 5ml each was collected at the baseline and on the 21st day and sent immediately to the microbiological laboratory for semi-quantitative analysis. Clinical parameters such as Gingival Index (GI), Plaque Index (PI), Papillary Bleeding Index (PBI), Periodontal Pocket depth (PPD), and Clinical attachment loss (CAL) were recorded at baseline, on the 7th day, and on the 21st day after phase – I therapy. Result: In both the groups a significant reduction in PI, GI, BI and CFU were observed between baseline and after 21 days of phase- I therapy (p<0.05) Conclusion: Using hyaluronic acid (HA) and chlorhexidine (CHX) mouthwashes alongside SRP improves periodontal health in patients with periodontitis, with HA having a stronger effect