The environment faces a significant threat due to the presence of agricultural residue that is difficult to break down. In order to address this issue, the current research focuses on decomposing harmful waste materials like feathers, human hairs, plastic, and epoxy. This decomposition process is facilitated by newly isolated endophytic bacteria in squash fruit. These bacteria, which form colonies in the intercellular space, were obtained from the melon fruit Benincasa hispida (Thunb) Cogn. A total of 22 bacterial colonies were isolated from the fruit using a highly alkaline medium with a pH of 14.0 and at a temperature of 37 °C. 12 bacterial populations were selected out of these colonies based on their growth conditions. These populations were named KARE_P1, KARE_P2, KARE_P3, KARE_P5, KARE_P8, KARE_P9, KARE_P11, KARE_P12, KARE_P14, KARE_P15, KARE_K1, KARE_K2, and KARE_W1. KARE_P3 and KARE_P8 were identified as non-pathogenic based on their 16S RNA sequence. Further characterization of these bacteria involved studying their growth under different conditions, including temperatures of 18°C, 37°C, and 55°C, and pH values of 5.0, 7.0, and 14.0. The protein extracted from these bacteria was analyzed using scanning electron microscopy (SEM) to assess their proteolytic and keratinolytic feather degradation capabilities. Additionally, a zymogram assay was conducted to evaluate their degradative potential.