Bengal bean or velvet bean botanically named as Mucuna prurience L. belongs to the Fabaceae family is an important ingredient in Ayurveda preparation. In Indian tradition medicinal plants play richest role and in modern economy as well. India is the oldest and most diverse culture in utilizing medicinal plants for curing various diseases. In addition to its medicinal use, it is also an excellent source of cover crop, food and green manure. The plant is pharmacologically investigated for curing various diseases like worms, dysentery, diarrhoea, sexual debility, muscular pain, menstrual disorder, diabetes, cancer and tuberculosis. Seed of the Mucuna prurience contains L-Dopa which is used in ayurvedic drugs and medicine. Due to its high pharmacological usage and commercial value, the variety is now in the endangered category. so as to meet the demands of mankind and pharmaceutical company there is a need to develop a reliable protocol for micropropagation of Mucuna prurience. Hence, the purpose of the present investigation is to develop an efficient in-vitro regeneration method for this important medicinal plant of India, Mucuna prurience. 3% sucrose in Murashige and Skoog ‘s media with 5.8 -6.0 PH was used for organogenesis. A range of various conc. of different types of auxins (IAA, IBA, and NAA) alone and in different combinations with BAP (6, benzyl amino purine) was studied for shoot regeneration with cotyledon & cotyledonary node as an explant. Shoot apex explant proved to be efficient for multiple shooting. The reading was taken after 20-25 days of incubation. Out of 3 auxins (IAA, IBA, NAA) used in Murashige and Skoog ‘s media, NAA was found to be most effective. Moderate 5 mg/l conc. of NAA alone showed optimum 95% shoot induction. In present study low to moderate (1-2.5 mg/l) conc. of NAA in combination with high conc. (10 mg/l of BAP) proved to be highly effective for shoot regeneration. The highest shoot proliferation i.e., 92% was found in 2.5 mg/l NAA / 10mg/l BAP. Low conc. of NAA i.e., 1mg/l in combination with high conc. i.e., 5 mg/l BAP also showed good response 90% of multiple shoot induction. Higher and moderate conc. i.e., 5 & 10 mg/l IBA in half strength MS was proved to be the most effective for root regeneration. The plantlets were acclimatized & transferred to small plastic cups filled with sterilized sand & soil mixture (1:1), where 90% of the plantlets showed effective and normal growth. All plantlets were irrigated with Knop ‘s nutrient solution. After proper hardening all plantlets were shifted to earthen pot for normal growth. Thus, the present investigation provides high and reliable protocol for in vitro micro propagation of Mucuna prurience